目的 采用网络药理学方法分析香连丸活性成分、靶点、通路及生物过程,探讨其药效物质基础及作用机制。方法 运用TCMSP数据库及BATMAN-TCM分析平台获取香连丸活性成分及对应靶点,采用Cytoscape 3.5.1软件构建香连丸活性成分-靶点网络,运用STRING平台构建靶蛋白相互作用网络,利用BINGO和MCODE插件对靶基因进行GO生物过程富集和聚类分析,借助生物学信息注释数据库(DAVID)对靶点进行KEGG信号通路分析。结果 香连丸11个活性成分作用于291个潜在靶点,参与炎症反应、白三烯代谢、脂质代谢、凋亡、平滑肌收缩、血小板活化等18个主要GO生物过程调节子簇;涉及炎症、代谢、信号转导、癌症、消化液分泌、心血管系统等7大类33条信号通路。结论 预测了香连丸在胃肠炎症、糖尿病、癌症以及心血管疾病上的不同治疗价值,以及对应的药效物质基础和分子机制,为香连丸临床应用拓展提供了新的线索。
Abstract
OBJECTIVE To analyze active ingredients,targets,pathways and biological processes of xianglian pills by means of network pharmacology and explore its pharmacodynamics material base and mechanism of action.METHODS Active ingredients and corresponding targets of xianglian pills were obtained by using TCMSP database and BATMAN-TCM analytical platform,the network of xianglian pills active ingredients-targets was constructed by using Cytoscape 3.5.1 software, using the STRING platform to build protein-protein interaction; GO bioprocess enrichment and clustering analysis of target genes were finished by using BINGO and MCODE plug-ins; KEGG signaling pathways of target genes were analyzed by means of DAVID database.RESULTS Eleven active ingredients in xianglian pills act on 291 potential targets. these targets participate in eighteen major GO biological process adjustment sub-clusters of the inflammatory response, leukotriene metabolism, lipid metabolism, apoptosis, smooth muscle contraction and platelet activation etc. involve in thirty-three signal pathways of seven categories: inflammation, metabolism,signal transduction, cancer, secretion of digestive juices, ardiovascular system etc.CONCLUSION The different therapeutic value, pharmacodynamic substances and molecular mechanism of Xianglian pills in the treatment of inflammation,diabetes,cancer and cardiovascular diseases have been preliminarily elucidated, which provides new clues for the development of its clinical application.
关键词
香连丸 /
网络药理学 /
聚类分析 /
蛋白相互作用 /
通路
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Key words
xianglian pills /
network pharmacology /
cluster analysis /
protein-protein interaction /
pathways
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